Since NO plays an important role in pain [17] and participates in release of migraine-related factors, including substance P and prostaglandin E2 [18] which are involved in pain [19, 20], the aim of this study was to study the role of NO system in migraine-induced alterations in anxiety-like behaviors, motor function and passive avoidance learning and memory. This evidence concerns the gene TAC1 and migraine disorder.