The generation of mutant homology models as described in the Materials and Methods section and structural analysis of the mutants versus their respective wild types revealed variant-induced alteration in local hydrogen bond number and bond length among the interacting amino acid residues for the variants; 10 in ROBO1, 10 in ROBO4 and 14 in SLIT2 from lung cancer dataset. The gene discussed is ROBO1; the disease is lung cancer.