About 13% of ROBO1, 13% of ROBO4, and 19% of SLIT2 variants of non-lung cancer dataset were predicted to be "possibly damaging" by PolyPhen2. Similarly, about 53% of ROBO1, 50% of ROBO4, and 46% of SLIT2 variants of lung cancer dataset to be "possibly damaging", while 45% of ROBO1, 50% of ROBO4, and 43% of SLIT2 variants of non-lung cancer dataset were predicted to be "probably damaging" by PolyPhen2. SNPs&GO44 predicted about 35% of ROBO1, 35% of ROBO4, and 32% of SLIT2 variants of lung cancer dataset to be "Disease-related". Here, ROBO1 is linked to lung carcinoma.