Expression of the tumour suppressor Neurofibromatosis Type 1 (NF1) was greatly reduced in JK16 and 83Mes GSC lines (Fig. 4C), corroborating they represent the mesenchymal subtype because hemizygous deletions of a region at 17q11.2, containing the NF1 gene, is a hallmark of this GBM subtype4,5. Here, NF1 is linked to glioblastoma.