ESCC cells treatment with 50 μM of IOX1 increased H3K9me2 and slightly alter H3K27me3 levels (Fig. 3a, b), whereas decreased protein expression of KDM3A and KDM6B was also apparent in both in vitro hypoxic conditions (Fig. 3b). Here, KDM3A is linked to esophageal squamous cell carcinoma.