Noteworthy, Illumina called two additional unique CDKN2A variants (NM_001195132: c.35C > T (p.Ser12Leu) and c.35delC (p.Ser12TrpfsTer14)) in one tumor sample (ID #10), but both variants had a coverage of 108x and were thus excluded by our detection limit (Additional File 4). This evidence concerns the gene CDKN2A and neoplasm.