In this regard, we co-transfected hPSCs with pEF-XMAS1xStop/2xStop and pEF-ABEmax along with a pDT-sgRNA targeting genomic Site-1 or single base pair changes in AKAP9 and PSEN1 that have been previously associated with increased risks of developing Alzheimer’s disease (AD) [28, 29]. The gene discussed is PSEN1; the disease is early-onset autosomal dominant Alzheimer disease.