Western blot (Figure 6C, 6D, Figure 6G, 6H) and quantitative RT-PCR (Figure 6B, 6F) results indicated that FX5 treatment suppressed protein level of HNF4α, mRNA levels of GR and HNF4α, as well as expression level of miR122-5p in db/db or HFD/STZ induced T2DM mice. This evidence concerns the gene HNF4A and type 2 diabetes mellitus.