LETM1 and colorectal carcinoma: Therefore, to examine whether autophagy was involved in LETM1‐dependent proliferation and stemness in CRC cells, we treated the cells with the autophagy inhibitor 3‐methyladenine (3‐MA) or the autophagy activator rapamycin (RAPA) for 24 hours after transfection and evaluated cell death and proliferation.