In addition, the mRNA level of ASIC1 in spinal dorsal horn was not altered compared with CON rats (Figure 1E, P > 0.05, n = 5 rats for CON and n = 4 rats for PMS, two‐sample t‐test), nor the ASIC2 expression (Figure 1F, P > 0.05, n = 5 rats for CON and n = 4 rats for PMS, two‐sample t‐test). This evidence concerns the gene ASIC1 and premenstrual tension.