We confirmed our gating strategy for type II NKT cells using CD1d-/- mice; most of the TET-CD8α-CD4+NK1.1+TCRβ+ cells were CD1d-restricted as the percentage and total number of this population was reduced in CD1d-/- mice after infection (Supplementary Figure 4BandFigure 3B). Here, CD1D is linked to infection.