To test this hypothesis, we isolated EVs from the CM of classical miR-375-expressing MCC cell lines (WaGa, PeTa, MKL-1, and UM-MCC-13); these EVs were typically sized between 50 and 100 nm (Fig. 2c), and positive for CD63 and Tsg101, two common EV markers, and negative for Calnexin, a marker for the endoplasmic reticulum as identified by immunoblot (Fig. 2d). The gene discussed is CANX; the disease is Merkel cell skin cancer.