To investigate the influence of SEMGs on metabolic enzymes and energy metabolism in several cancer models, we have established H1299 (non-small lung adenocarcinoma), MDA-MB-231 (breast carcinoma) and Mia-Paca2 (pancreatic adenocarcinoma) cell lines with stable overexpression of 3×Flag-tagged SEMG1 or SEMG2. This evidence concerns the gene SEMG1 and cancer.