In contrast with the bioinformatics data, Fig. 1C shows that SEMG2 was expressed at the protein level in almost all cell lines tested excluding two lines of pancreatic cancer whereas SEMG1 was detected in only three cell lines—MCF7 (breast carcinoma), U2OS (osteosarcoma) and H520 (NSCLC). This evidence concerns the gene SEMG2 and osteosarcoma.