Our a priori hypothesis was that increased c-Myc expression leads to the progression of pre-malignant plasma cells in MGUS to malignant plasma cells in MM with a resultant increase in glutamine anaplerosis into the TCA cycle that is facilitated by the overexpression of GLS1 that converts glutamine to glutamate as well as by overexpression of the glutamine cell membrane transporters ASCT2 and SN2. This evidence concerns the gene GLS and Miyoshi myopathy.