However, HCT-116 cells treated with ATP13A2 targeting siRNA showed no increase in the number LC3-GFP puncta after bafilomycin A1 treatment; this was rescued by the overexpression of ATP13A2 in colon cancer cells, indicating that ATP13A2 knockdown decreased basal autophagic flux (Fig. 5d). This evidence concerns the gene ATP13A2 and malignant colon neoplasm.