MAP1LC3A and malignant colon neoplasm: However, HCT-116 cells treated with ATP13A2 targeting siRNA showed no increase in the number LC3-GFP puncta after bafilomycin A1 treatment; this was rescued by the overexpression of ATP13A2 in colon cancer cells, indicating that ATP13A2 knockdown decreased basal autophagic flux (Fig. 5d).