EIF5A2 expression was up-regulated in HCC tissues, especially in DOX-resistant tissues, (Fig. 5d, e) and cell lines (Fig. 5f, g) at mRNA and protein levels, and also elevated in Huh7/DOX and HCCLM3/DOX cells compared with the parental cells (Fig. 5h, i). This evidence concerns the gene EIF5A2 and hepatocellular carcinoma.