In the patient 10–33 a 6-year-old boy with a reduction of visual acuity to the level of counting fingers, night blindness, astigmatism, and myopia NGS panel for LCA genes revealed a homozygous substitution c.304G>T in exon 4 (p.Glu102*) in the RPE65 gene. This evidence concerns the gene RPE65 and Astigmatism.