SPP1 and congenital bilateral aplasia of vas deferens from CFTR mutation: Wild-type diet-based mouse CAVD models have previously demonstrated deposition of monocyte-macrophages, lipids, and lipoproteins [16, 17], but to our knowledge no such diet-based models exhibit early calcification as demonstrated by positive Alizarin Red S (ARS) staining, as well as positive osteopontin and Runt-related transcription factor 2 (RUNX2) expression [14,17].