Our results show that NAFLD significantly affects trace element (Zn, Cl, Fe, Mg, Zn) serum levels in two experimental animals model, in agreement with precedent studies [4]; moreover, using serum samples obtained from MCD and Obese Zucker rats, the current study documented the upregulation of IL-1beta, IL-6, TNF-alpha as reported by Ji et al. [14], as well as the existence of significant correlations between these trace elements and pro-inflammation markers such as: TNF-alpha, IL-1beta and IL-6. Here, IL6 is linked to metabolic dysfunction-associated steatotic liver disease.