Since employing PHH may be resource-prohibitive for many researchers, hepatocellular carcinoma (HCC) cell lines that express more THRB than THRA, such as Huh-7 cells (Fig 2B), are suitable alternatives and have been routinely used as in vitro models for liver diseases such as NAFLD [47,48]. The gene discussed is THRB; the disease is hepatocellular carcinoma.