In line with this emergency requirement, our study proved that the transcription of hTERT in CRC is strictly controlled by SPT6 and SPT6/hTERT signaling axis serves as a potential therapeutic target in CRC evidenced by the direct binding of SPT6 at the promoter region of hTERT resulting in the transcription initiation and translation changes of hTERT and subsequent influence on the function of colon cancer cells in vitro and in vivo. Here, SUPT6H is linked to malignant colon neoplasm.