We also detected the change of cytochrome C release and the expressions of apoptosis‐related markers, and the results showed that SPT6 silencing led to more release of cytochrome C from mitochondria to cytoplasm and more accumulation of cleaved Caspase 9, Caspase 3, and PARP in colon cancer cells (Fig. S2A, Fig. 3C), confirming the promotability of apoptosis upon SPT6 knockdown. Here, CYCS is linked to malignant colon neoplasm.