Following our prespecified selection criteria, data analysis of the HM450K assay revealed that ZIC4, CASP8, RAB32, RARA, and TRAF6 were the five most common genes with more hypomethylated CpG sites located within the promoter region and 5′ UTR in NTD cases than in controls (Supplementary Material, Supplementary Table 4). The gene discussed is CASP8; the disease is neural tube defect.