We found the expression of CHRNB4 was higher in cancer with SCCs including CECC, LUSC, HNSC, and ESCC than in adenocarcinomas (AC) such as STAD, LUAD, PAAD, COAD, READ, and EAC (Figures 2D, E), suggesting that CHRNB4 was significantly and specifically upregulated in SCCs compared to ACs. The gene discussed is CHRNB4; the disease is esophageal squamous cell carcinoma.