SYCN and familial pancreatic carcinoma: Shalini Makawita et al. demonstrated islet-derived 1 beta (REG1B) and syncollin (SYCN) as candidate biomarkers of PDAC in 2011 and 2013, respectively, by using two-dimensional liquid chromatography/tandem mass spectrometry (LC-MS/MS) for proteomic analysis of six pancreatic juice samples from patients with PDAC and conditioned media from six pancreatic cancer cell lines and one normal pancreatic ductal epithelial cell line, followed by further validation of the two biomarkers by ELISA [40], [41].