Using BE3 and hA3A-BE3, Xie et al. (2019) simultaneously mutated the porcine RAG1, RAG2, and IL2RG or DMD, TYR, and LMNA triple gene in PFF cells with high efficiency, and subsequently generated a triple gene knockout pig model with immunodeficiency for applications in regenerative medicine. Here, IL2RG is linked to immunodeficiency disease.