Green fluorescent protein (GFP)-tagged N-mutHtt with a 109 amino acid-long repeat (N-mutHtt109Q-GFP) was co-expressed with mCherry or mCherry-Bri2 BRICHOS in human osteosarcoma U2OS cells and human cervix carcinoma HeLa cells and the localization of the proteins were examined by fluorescence microscopy. Here, ITM2B is linked to osteosarcoma.