Studies investigating combination treatment with lapatinib and ipatasertib in the PIK3CA-mut HER2+ breast cancer cell lines revealed that inhibition of both the MAPK and PI3K pathways is critical to inhibit proliferation in HER2+ breast cancer cell lines, and that expression levels of p4EBP1 were correlated with the antiproliferative effects of the inhibitors (Figs. 3a, 5a). Here, PIK3CA is linked to breast cancer.