In hepatocellular carcinoma cells (HuH7) which originally express CD44s rather than CD44v, silence of CD44 gene impaired the potential of spheroid formation and enhanced sensitivity to sorafenib and 5-fluorouracil (5‐FU), accompanied by remarkable downregulation of CSC-related genes including CD133 and EpCAM [19]. Here, CD44 is linked to hepatocellular carcinoma.