However, Ang-1 combined with VEGF induced non-leaky neovessels [22], and indeed, a preclinical report on a murine model of limb ischemia showed that VEGF-Ang1 chimeric gene or protein may promote neovascularization, which represented less leakiness, less tissue inflammation, less angioma-like formation, and better perfusion recovery as compared with VEGF alone [61]. This evidence concerns the gene ANGPT1 and limb ischemia.