These results suggest that CWS and CWS-Nano-CL induce the IRE1α-JNK-caspase 3 pathway as an apoptotic response to ER stress, which is mediated by AMPK activation and ROS generation in bladder cancer cells treated with CWS or CWS-Nano-CL. This evidence concerns the gene MAPK8 and urinary bladder carcinoma.