Herein, we researched the level of DLGAP1‐AS2 in CCA and assessed the impact of DLGAP1‐AS2 on the malignant biological behaviors of CCA cells in vitro, as well as its potential molecular mechanism, which laid a theoretical foundation for the application of DLGAP1‐AS2 as a therapeutic target of CCA. Here, DLGAP1 is linked to cholangiocarcinoma.