Notably, our assay of the impact of the TDP-43 pathogenic ALS mutation and overexpression in a neuroblastoma cell line, SH-SY5Y, revealed that the pathogenic A382T and G294V mutations in SH-SY5Y cells also affected the TDP-43 role controlling R-loop homeostasis, leading to a higher detection of genomic RNA-DNA hybrids, as detected by IF and DRIP-qPCR. The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.