Vpr expression (MOI = 1, Figure 2—figure supplement 1D) also suppressed cGAMP-mediated induction of endogenous ISG mRNA expression, measured by qRT-PCR for MxA, CXCL10, IFIT2, and viperin (Figure 2B) and inhibited cGAMP-induced CXCL10 secretion (Figure 2C; infection data to gauge MOI in Figure 2—figure supplement 1E). Here, CXCL10 is linked to infection.