In this study, the results of the gene chip analysis showed that the genes differentially sensitive and insensitive to CRT in NPC were associated with the immune microenvironment, including the immune response, T cell receptor signaling pathway, positive regulation of T cell proliferation, positive regulation of the innate immune response, Toll-like receptor TLR1:TLR2 signaling pathway, TCR signalosomes, and intracellular pH reduction. Here, TLR1 is linked to nasopharyngeal carcinoma.