DsDNA derived from plasma EVs of 20 AML patients at diagnosis and during treatment was examined for leukemia-specific mutations (e.g., NPM1, FLT3, WT1, GATA2, ETV6, ZRSR2, NOTCH1, NRAS, KIT, and PHF6). The gene discussed is FLT3; the disease is acute myeloid leukemia.