We confirmed that Lrp6 endocytosis was observed in SW480, a colon cancer cell line in which the absence of APC triggered, and APC reconstitution inhibited, Wnt signaling and Lrp6 endocytosis (Supplementary Movie S1); this is in agreement with recent results showing that APC is a major regulator of endocytosis16,29. Here, APC is linked to colonic neoplasm.