To screen for other pathogenic mutations affecting the HH signaling pathway, we performed NGS with the MiSeq sequencer using the TruSight One panel for familial NBCCS and identified the following mutations: PTCH1_c.1665T>C_p.N555N (exon 12), _c.2560+9G>C (intron 15), _c.2887+21G>A (intron 17), _c.3298_3299insAAG_p.1099_1100insE (exon 19), and _c.3944C>T_p.P1315L (exon 23). The gene discussed is PTCH1; the disease is nevoid basal cell carcinoma syndrome.