CASC9 and colorectal carcinoma: In parallel, we examined the expression levels of CASC9 in different cell lines using RT-qPCR and found that compared with those in the normal colonic epithelial cell line NCM460, CASC9 expression levels were markedly increased in CRC cell lines such as HT-29, SW480, SW620, HCT-116, and LoVo (Fig. 1d).