The data showed that 80% of Twist-1del/+ mice treated with 2 μM GSK-J4 exhibited open coronal sutures at postnatal day 20, whereas unilateral or bilateral coronal craniosynostosis was observed in 83% of the Twist-1del/+ mice treated with DMSO alone (Table 1 and Fig. 5d). This evidence concerns the gene TWIST1 and craniosynostosis.