KDM6A and craniosynostosis: Collectively, the present study demonstrated that GSK-J4 treatment effectively suppressed osteogenic differentiation of Twist-1del/+ calvarial cells in both sexes and whole calvaria explants in vitro, and prevented coronal suture craniosynostosis of Twist-1del/+ mice in vivo by inhibiting the enzymatic activity of aberrant Kdm6a and Kdm6b levels and thus recovering the level of H3K27me3 marks on osteogenic genes.