Furthermore, chidamide inhibited HDAC3, P-AKT, and CDK2 protein expression significantly, but increased P21 protein expression in a dose-dependent manner, while no significant changes in AKT protein levels were observed in HL60/ADR cells, K562/A02 cells, THP-1/ADR and primary AML cells (Fig. 5d, e; Figure S5C-D). This evidence concerns the gene HDAC3 and acute myeloid leukemia.