To determine the apoptotic effect of chidamide on anthracycline-resistant AML cells, HL60/ADR cells, K562/A02 and THP-1/ADR cells were treated with 0, 0.01, 0.05, 0.1 μM for 24 h or 48 h, respectively, and double-stained with Annexin V/PI before being analyzed using flow cytometry. Here, ANXA5 is linked to acute myeloid leukemia.