Furthermore, chidamide inhibited HDAC3, P-AKT, and CDK2 protein expression significantly, but increased P21 protein expression in a dose-dependent manner, while no significant changes in AKT protein levels were observed in HL60/ADR cells, K562/A02 cells, THP-1/ADR and primary AML cells (Fig. 5d, e; Figure S5C-D). The gene discussed is AKT1; the disease is acute myeloid leukemia.