Some predictive biomarkers including protein, DNA and mRNA, in melanoma immunotherapy, such as PDL1(protein), TMB (tumor mutational burden, DNA), B2m (β- microglobulin 2 gene, DNA, mRNA), associated with the tumor microenvironment and associated with the whole organism with melanoma, were candidates (reviewed in [3]). The gene discussed is B2M; the disease is neoplasm.