To inhibit autophagy, active mTORC1 in nutrient-rich conditions phosphorylates the Microphthalmia family of bHLH-LZ transcription factor (MiT/TFE) family member, transcription factor EB (TFEB), which, through the association with members of the YWHA (14-3-3) family of proteins, leads to cytoplasmic sequestration of this transcription factor away from the nucleus, where it transcribes a coordinated network of genes responsible for lysosomal biogenesis, lysosomal hydrolase production, and autophagy proteins [74]. This evidence concerns the gene TFEB and microphthalmia.