PEG13 and infection: To understand the regulatory role of Peg13, we packaged lentivirus for Peg13 over expression.The multiplicities of infection (MOI) of transfection were determined by fluorescent inverted phase contrast microscope and transduction with OE-Peg13 at a MOI of 10 increased Peg13 transcripts by 1.8 times, which was used for subsequent studies (Fig 4F).