The beneficial effect of linagliptin on cardiomyocyte stiffness in vivo was also accompanied by isoform switching from the stiff N2B isoform to the more compliant N2BA titin isoform in obese ZSF1 rats, most probably as a result of its positive effect on the components of the MetS, especially type 2 diabetes.45 Here, TTN is linked to type 2 diabetes mellitus.