The median levels of AFP and PIVKA-II in HBV-related HCC patients were 24.64 (IQR 4.38 ~ 528.82) and 334.08 (IQR 60.88 ~ 5095.10), while their concentrations of control group were 3.20 (IQR 1.90 ~ 9.60) and 22.17 (IQR 17.59 ~ 30.05) respectively. The gene discussed is AFP; the disease is hepatocellular carcinoma.