To confirm the association between SKP1 and CRC stemness, we established SKP1-overexpressing HCT-116 and #1 primary CRC cells by lentivirus delivery system (Additional file 2: Figure S1) and examined the properties related to CRC-SCs, including the capacity of self-renewal, expression of related markers as well as tumorigenesis. The gene discussed is SKP1; the disease is colorectal carcinoma.