As expected, SKP1 depletion impaired the stemness of CRC cells reflected by reduced sphere-forming capacity (Fig. 3a), the frequency of sphere-forming cells (Fig. 3b), and the mRNA levels of CRC-SCs markers (SOX2, ALDH1, CD44 and CD133) (Fig. 3c). The gene discussed is SOX2; the disease is colorectal carcinoma.