In order to prove the relationship between SLC39A1 and tumor immune infiltration, we employed the ESTIMATE algorithm to estimate stroma cell and immune cell infiltration scores in gliomas, and calculated the correlation between them and SLC39A1, finding that SLC39A1 expression was significantly positively correlated with ImmuneScore and StromalScore, and significantly negatively correlated with TumorPurity (Fig. 9a). The gene discussed is SLC39A1; the disease is glioma.