AFP and neoplasm: Finally, immunohistochemical results showed that compared with the control group, the number of AFP-positive cells in tumor tissues decreased in the circSMYD4 + MC group and increased in the NC + M group, and were significantly bigger in the circSMYD4 + M group than in the circSMYD4 + MC group (200 × magnification, Scale = 100 μm, Fig. 8c).