In addition, in the subgroup analysis stratified by the detection method, RASSF1A gene promoter hypermethylation was evidently related to HCC risk (MSP: OR = 7.30, 95% CI = 5.17–10.29, P < 0.001; others: OR = 6.20, 95% CI = 3.13–12.30, P < 0.001). Here, RASSF1 is linked to hepatocellular carcinoma.