Then we further investigate whether parthenolide could suppress EGFR-mediated MEK/ERK and PI3K/AKT cascades in vivo, results showed that parthenolide treated mice exhibited prominently increased apoptosis and suppressed cell proliferation as indicated by cleaved caspase-3 and Ki-67 immunostaining, as well as decreased cellularity as measured by HE staining, supporting the observations on the inhibition of tumor growth. This evidence concerns the gene AKT1 and neoplasm.