To explore whether miR-1305 facilitated cell viability, proliferation and adhesion while inhibiting the apoptosis of ESCC cells by targeting TXNRD1, we transfected si-TXNRD1, miR-1305 inhibitor or si-TXNRD1 + miR-1305 inhibitor in KYSE450 and KYSE510 cells. The gene discussed is TXNRD1; the disease is esophageal squamous cell carcinoma.